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Riboflavin

Vitamin B2, Lactoflavin, Riboflavin-5-phosphate, E101, Vitamin B-2
Riboflavin, also known as vitamin B2, is a water-soluble vitamin used in eye care as a dye in certain diagnostic techniques and treatments for the cornea.

What is riboflavin?

Riboflavin is a water-soluble B-complex vitamin, also known as vitamin B2. In eye care, riboflavin is mainly used as a dye that makes the cornea visible under ultraviolet light. This property makes it suitable for diagnostic purposes and for certain treatments carried out by an ophthalmologist.

Riboflavin is yellow to orange in colour and dissolves well in water. When applied to the surface of the eye, it may cause temporary slight discolouration. The substance is normally absorbed by the body through the diet and plays a role in various metabolic processes.

In ophthalmology, riboflavin is sometimes used in combination with UV light in a procedure known as cross-linking. This technique is used by ophthalmologists to treat certain corneal conditions. Riboflavin eye drops are available on prescription only and must be used strictly as directed by an ophthalmologist.

Good to know

Use in eye care

In eye care, riboflavin is mainly used in diagnostic examinations and in specific treatments of the cornea. The substance is administered as eye drops and makes certain structures of the eye more visible under special lighting. This helps the ophthalmologist to assess the condition of the cornea.

For certain corneal conditions, an ophthalmologist may use riboflavin in combination with UV light. This procedure is known as cross-linking and is intended to strengthen the structure of the cornea. It is carried out exclusively in a clinical setting under the supervision of an ophthalmologist.

Availability

Riboflavin eye drops are available on prescription only and are dispensed only on the advice of an ophthalmologist. You cannot purchase or use these drops yourself without medical supervision. The dosage and method of use are determined by the ophthalmologist based on the specific situation.

Side effects and precautions

Riboflavin may temporarily cause a yellow discolouration of the surface of the eye, which will disappear over time. Mild irritation or a burning sensation may occur during and after use. If you experience any unusual symptoms after using riboflavin drops, or if the symptoms persist, please contact your ophthalmologist.

As riboflavin is used in eye care in combination with UV light, it is important that the procedure is carried out by an experienced ophthalmologist. The treatment requires specific equipment and expertise to ensure it is carried out safely and in accordance with protocol.

Frequently asked questions

No, riboflavin eye drops are available on prescription only and are dispensed only on the advice of an ophthalmologist. These drops are used in specific medical procedures and diagnostics, and are not intended for self-medication. Your ophthalmologist will determine whether and when riboflavin is suitable for your situation.

Riboflavin has the property of reacting to UV light, which means it can be used in a technique known as cross-linking. In this procedure, the ophthalmologist applies riboflavin to the cornea and then exposes it to UV light. This is intended to strengthen the structure of the cornea in certain conditions.

Yes, riboflavin is yellow to orange in colour and may cause temporary slight discolouration of the surface of the eye. This discolouration is normal and will disappear on its own over time. Your ophthalmologist will inform you in advance about what to expect during and after treatment.

Yes, riboflavin is the chemical name for vitamin B2. In eye care, however, riboflavin is not used as a vitamin supplement, but as an active ingredient in diagnostic and therapeutic procedures. The form and concentration used by the ophthalmologist differ from the vitamin B2 you obtain through your diet or supplements.

Author & updates

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Publication date: 13 July 2026
Last updated: 13 July 2026